Composite

Part:BBa_K311004:Design

Designed by: Matthew Adams, Swati Choudhary, Anthony Goering, Rachel Farr, Ethan Johnson, Annie Kathuria, Poonam Srivastava, Ian Windsor   Group: iGEM10_Minnesota   (2010-10-15)


Complement of Ethanolamine Utilization Microcompartment proteins from Salmonella LT2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 476
    Illegal NotI site found at 1386
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
    Illegal BglII site found at 689
    Illegal BglII site found at 1599
    Illegal XhoI site found at 484
    Illegal XhoI site found at 1394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 901


Design Notes

The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. The genes were ligated into a modified pUC biobrick vector containing a constitutive promoter. The genes were taking in their native operons and ligated sequential according to size.


Source

Salmonella enterica LT2 genomic DNA

References